Atlas of Subtiligase-Captured Extracellular N Termini
ASCENT is a database of cell surface N termini captured using a subtiligase-transmembrane domain fusion protein expressed in live cells. Use the links below to browse the datasets or to search for a protein of interest.
Subtiligase-TM modifies cell surface N termini with a peptide containing a biotin group for affinity enrichment, a TEV protease cleavage site for selective elution, and an aminobutyric acid mass tag for positive identification of bona fide substrates. Following selective isolation of ligated peptides and sequencing by LC-MS/MS, the positions of N termini generated by proteolytic cleavage can be determined with single amino acid resolution.