ASCENT

RPN1_HUMAN
P04843 - Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

FUNCTION: Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. {ECO:0000250|UniProtKB:E2RQ08, ECO:0000250|UniProtKB:Q9GMB0, ECO:0000305}.

SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000250|UniProtKB:E2RQ08, ECO:0000250|UniProtKB:Q9GMB0}. Endoplasmic reticulum membrane; Single-pass type I membrane protein {ECO:0000305}. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

CLEAVAGE EVENTS OBSERVED:

PLOT OF CLEAVAGE EVENTS:

CADH2_HUMAN
P19022 - Cadherin-2

FUNCTION: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity). {ECO:0000250}.

SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P15116}; Single-pass type I membrane protein {ECO:0000255}. Cell membrane, sarcolemma {ECO:0000250|UniProtKB:P15116}. Cell junction {ECO:0000269|PubMed:28169360}. Cell surface {ECO:0000250|UniProtKB:P15116}. Note=Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes. {ECO:0000250|UniProtKB:P15116}.

CLEAVAGE EVENTS OBSERVED:

PLOT OF CLEAVAGE EVENTS:

TXND5_HUMAN
Q8NBS9 - Thioredoxin domain-containing protein 5

FUNCTION: Possesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide- isomerase deficiency in yeast (By similarity). {ECO:0000250}.

SUBCELLULAR LOCATION: Endoplasmic reticulum lumen {ECO:0000255|PROSITE-ProRule:PRU10138}.

CLEAVAGE EVENTS OBSERVED:

PLOT OF CLEAVAGE EVENTS:

TM9S2_HUMAN
Q99805 - Transmembrane 9 superfamily member 2

FUNCTION: In the intracellular compartments, may function as a channel or small molecule transporter.

SUBCELLULAR LOCATION: Endosome membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.

CLEAVAGE EVENTS OBSERVED:

PLOT OF CLEAVAGE EVENTS:

CNPY4_HUMAN
Q8N129 - Protein canopy homolog 4

FUNCTION: Plays a role in the regulation of the cell surface expression of TLR4. {ECO:0000269|PubMed:16338228}.

SUBCELLULAR LOCATION: Secreted {ECO:0000305}.

CLEAVAGE EVENTS OBSERVED:

PLOT OF CLEAVAGE EVENTS:

S39AA_HUMAN
Q9ULF5 - Zinc transporter ZIP10

FUNCTION: May act as a zinc-influx transporter. {ECO:0000269|PubMed:17359283}.

SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.

CLEAVAGE EVENTS OBSERVED:

PLOT OF CLEAVAGE EVENTS:

TMX4_HUMAN
Q9H1E5 - Thioredoxin-related transmembrane protein 4

SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}.

CLEAVAGE EVENTS OBSERVED:

PLOT OF CLEAVAGE EVENTS:

S39A7_HUMAN
Q92504 - Zinc transporter SLC39A7

FUNCTION: Zinc transporter, that transports Zn(2+) from the endoplasmic reticulum/Golgi apparatus to the cytosol. Transport is stimulated by growth factors, such as EGF, and Ca(2+), as well as by exogenous Zn(2+). {ECO:0000269|PubMed:14525538, ECO:0000269|PubMed:15705588, ECO:0000269|PubMed:22317921}.

SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, cis-Golgi network membrane.

CLEAVAGE EVENTS OBSERVED:

PLOT OF CLEAVAGE EVENTS:

GLU2B_HUMAN
P14314 - Glucosidase 2 subunit beta

FUNCTION: Regulatory subunit of glucosidase II. {ECO:0000269|PubMed:10929008}.

SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000255|PROSITE- ProRule:PRU10138}.

CLEAVAGE EVENTS OBSERVED:

PLOT OF CLEAVAGE EVENTS:

GPI8_HUMAN
Q92643 - GPI-anchor transamidase

FUNCTION: Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein.

SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:11483512}; Single-pass type I membrane protein {ECO:0000269|PubMed:11483512}.

CLEAVAGE EVENTS OBSERVED:

PLOT OF CLEAVAGE EVENTS:

LRIG2_HUMAN
O94898 - Leucine-rich repeats and immunoglobulin-like domains protein 2

SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15145052}; Single-pass type I membrane protein {ECO:0000269|PubMed:15145052}. Cytoplasm {ECO:0000269|PubMed:15145052}.

CLEAVAGE EVENTS OBSERVED:

PLOT OF CLEAVAGE EVENTS:

TR10B_HUMAN
O14763 - Tumor necrosis factor receptor superfamily member 10B

FUNCTION: Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed:10549288). The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. {ECO:0000269|PubMed:10542098, ECO:0000269|PubMed:10549288, ECO:0000269|PubMed:15322075}.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

CLEAVAGE EVENTS OBSERVED:

PLOT OF CLEAVAGE EVENTS:

SELT_HUMAN
P62341 - Selenoprotein T {ECO:0000303|PubMed:27645994}

FUNCTION: Selenoprotein with thioredoxin reductase-like oxidoreductase activity (By similarity). Protects dopaminergic neurons against oxidative stress ans cell death (PubMed:26866473). Involved in ADCYAP1/PACAP-induced calcium mobilization and neuroendocrine secretion (By similarity). Plays a role in fibroblast anchorage and redox regulation (By similarity). In gastric smooth muscle, modulates the contraction processes through the regulation of calcium release and MYLK activation (By similarity). In pancreatic islets, involved in the control of glucose homeostasis, contributes to prolonged ADCYAP1/PACAP- induced insulin secretion (By similarity). {ECO:0000250|UniProtKB:P62342, ECO:0000250|UniProtKB:Q1H5H1, ECO:0000269|PubMed:26866473}.

SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q1H5H1}; Single-pass membrane protein {ECO:0000255}.

CLEAVAGE EVENTS OBSERVED:

PLOT OF CLEAVAGE EVENTS:

NOTC2_HUMAN
Q04721 - Neurogenic locus notch homolog protein 2

FUNCTION: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737). {ECO:0000250|UniProtKB:O35516, ECO:0000269|PubMed:21378985, ECO:0000269|PubMed:21378989, ECO:0000269|PubMed:25985737}.

SUBCELLULAR LOCATION: Notch 2 extracellular truncation: Cell membrane {ECO:0000269|PubMed:9244302}; Single-pass type I membrane protein {ECO:0000269|PubMed:9244302}.

FUNCTION: Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed:11489913). The association of the PARD6- PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha- L/beta-2 involved in memory T-cell and neutrophil transmigration (PubMed:11812992). Involved in platelet activation (PubMed:10753840). {ECO:0000250|UniProtKB:O88792, ECO:0000269|PubMed:10753840, ECO:0000269|PubMed:11489913, ECO:0000269|PubMed:11812992}.

FUNCTION: (Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1. {ECO:0000269|PubMed:11239401}.

FUNCTION: (Microbial infection) Acts as a receptor for Human Rotavirus strain Wa. {ECO:0000269|PubMed:25481868}.

SUBCELLULAR LOCATION: Cell junction, tight junction {ECO:0000269|PubMed:11171323}. Cell membrane {ECO:0000269|PubMed:11171323}; Single-pass type I membrane protein {ECO:0000269|PubMed:11171323}. Note=Localized at tight junctions of both epithelial and endothelial cells. {ECO:0000269|PubMed:11171323}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
JAM1_HUMAN	SVTVHSSEPEVR	28	LALG | SVTV

PLOT OF CLEAVAGE EVENTS:

EMC7_HUMAN
Q9NPA0 - ER membrane protein complex subunit 7

SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:22119785}; Single-pass type I membrane protein {ECO:0000269|PubMed:22119785}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
EMC7_HUMAN	SEVPGAAAEGSGGSGVGIGDR	24	DVQS | SEVP

PLOT OF CLEAVAGE EVENTS:

TGO1_HUMAN
Q5JRA6 - Transport and Golgi organization protein 1 homolog {ECO:0000305}

FUNCTION: Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum (PubMed:27138255). {ECO:0000269|PubMed:19269366, ECO:0000269|PubMed:27138255}.

SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:19269366, ECO:0000269|PubMed:21525241}; Single-pass membrane protein {ECO:0000269|PubMed:19269366}. Note=Localizes at endoplasmic reticulum exit sites. After loading of COL7A1 into transport carriers, it is not incorporated into COPII carriers and remains in the endoplasmic reticulum membrane. {ECO:0000269|PubMed:19269366, ECO:0000269|PubMed:21525241}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
TGO1_HUMAN	GLAGEPEGELSKEDHENTEK	866	LKTS | GLAG

PLOT OF CLEAVAGE EVENTS:

SDC1_HUMAN
P18827 - Syndecan-1

FUNCTION: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed:22660413). {ECO:0000269|PubMed:22660413}.

SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass type I membrane protein {ECO:0000255}. Secreted {ECO:0000269|PubMed:9169435}. Secreted, exosome {ECO:0000269|PubMed:22660413}. Note=Shedding of the ectodomain produces a soluble form. {ECO:0000269|PubMed:9169435}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
SDC1_HUMAN	STSTLPAGEGPK	89	ATAA | STST

PLOT OF CLEAVAGE EVENTS:

STIM1_HUMAN
Q13586 - Stromal interaction molecule 1

FUNCTION: Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores (PubMed:15866891, PubMed:16005298, PubMed:16208375, PubMed:16537481, PubMed:16733527, PubMed:16766533, PubMed:16807233, PubMed:18854159, PubMed:19249086, PubMed:22464749, PubMed:24069340, PubMed:24351972, PubMed:24591628, PubMed:26322679, PubMed:25326555, PubMed:28219928). Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit ORAI1 (PubMed:16208375, PubMed:16537481). Involved in enamel formation (PubMed:24621671). Activated following interaction with TMEM110/STIMATE, leading to promote STIM1 conformational switch (PubMed:26322679). {ECO:0000269|PubMed:15866891, ECO:0000269|PubMed:16005298, ECO:0000269|PubMed:16208375, ECO:0000269|PubMed:16537481, ECO:0000269|PubMed:16733527, ECO:0000269|PubMed:16766533, ECO:0000269|PubMed:16807233, ECO:0000269|PubMed:18854159, ECO:0000269|PubMed:19249086, ECO:0000269|PubMed:22464749, ECO:0000269|PubMed:24069340, ECO:0000269|PubMed:24351972, ECO:0000269|PubMed:24591628, ECO:0000269|PubMed:24621671, ECO:0000269|PubMed:25326555, ECO:0000269|PubMed:26322679, ECO:0000269|PubMed:28219928}.

SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein {ECO:0000269|PubMed:11004585, ECO:0000269|PubMed:16005298, ECO:0000269|PubMed:16208375, ECO:0000269|PubMed:18854159, ECO:0000269|PubMed:19249086, ECO:0000269|PubMed:28219928}. Endoplasmic reticulum membrane; Single-pass type I membrane protein {ECO:0000269|PubMed:16005298, ECO:0000269|PubMed:16208375, ECO:0000269|PubMed:18854159, ECO:0000269|PubMed:19249086, ECO:0000269|PubMed:26322679}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:19632184}. Sarcoplasmic reticulum {ECO:0000269|PubMed:25326555}. Note=Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium and is detected at punctae corresponding to junctions between the endoplasmic reticulum and the cell membrane (PubMed:19249086, PubMed:16005298, PubMed:16208375, PubMed:18854159). Associated with the microtubule network at the growing distal tip of microtubules (PubMed:19632184). {ECO:0000269|PubMed:16005298, ECO:0000269|PubMed:16208375, ECO:0000269|PubMed:18854159, ECO:0000269|PubMed:19249086, ECO:0000269|PubMed:19632184}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
STIM1_HUMAN	SSGANSEESTAAEFCR	35	ATGT | SSGA

PLOT OF CLEAVAGE EVENTS:

OCLN_HUMAN
Q16625 - Occludin

FUNCTION: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. {ECO:0000269|PubMed:19114660}.

SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:19017651, ECO:0000269|PubMed:19114660, ECO:0000269|PubMed:9175707}; Multi- pass membrane protein {ECO:0000255}. Cell junction, tight junction {ECO:0000269|PubMed:10523508, ECO:0000269|PubMed:19017651, ECO:0000269|PubMed:19114660, ECO:0000269|PubMed:19332538, ECO:0000269|PubMed:9175707}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
OCLN_HUMAN	GYGYGYGGYTDPR	123	GYGY | GYGY

PLOT OF CLEAVAGE EVENTS:

CD99_HUMAN
P14209 - CD99 antigen

FUNCTION: Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity). {ECO:0000250}.

SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
CD99_HUMAN	SFSDADLADGVSGGEGK	89	SSSG | SFSD

PLOT OF CLEAVAGE EVENTS:

GPC3_HUMAN
P51654 - Glypican-3

FUNCTION: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. {ECO:0000269|PubMed:17549790}.

SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Lipid-anchor, GPI-anchor {ECO:0000250}; Extracellular side {ECO:0000250}.

SUBCELLULAR LOCATION: Secreted glypican-3: Secreted, extracellular space {ECO:0000250}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
GPC3_HUMAN	HVEHEETLSSR	377	LKVA | HVEH

PLOT OF CLEAVAGE EVENTS:

FKB14_HUMAN
Q9NWM8 - Peptidyl-prolyl cis-trans isomerase FKBP14

FUNCTION: PPIases accelerate the folding of proteins during protein synthesis.

SUBCELLULAR LOCATION: Endoplasmic reticulum lumen {ECO:0000255|PROSITE-ProRule:PRU10138, ECO:0000269|PubMed:22265013}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
FKB14_HUMAN	ALIPEPEVK	20	SLIG | ALIP

PLOT OF CLEAVAGE EVENTS:

TXD15_HUMAN
Q96J42 - Thioredoxin domain-containing protein 15

SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
TXD15_HUMAN	SVIPGEAEDK	95	MVML | SVIP

PLOT OF CLEAVAGE EVENTS:

DSC3_HUMAN
Q14574 - Desmocollin-3

FUNCTION: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms.

SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Cell junction, desmosome.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
DSC3_HUMAN	TADGYSADLPLPLPIR	217	AYAS | TADG

PLOT OF CLEAVAGE EVENTS:

LMA2L_HUMAN
Q9H0V9 - VIP36-like protein

FUNCTION: May be involved in the regulation of export from the endoplasmic reticulum of a subset of glycoproteins. May function as a regulator of ERGIC-53. {ECO:0000269|PubMed:12878160}.

SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Note=Predominantly found in the endoplasmic reticulum. Partly found in the Golgi.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
LMA2L_HUMAN	GQTFEYLK	45	QVGA | GQTF
LMA2L_HUMAN	GQTFEYLKR	45	QVGA | GQTF

PLOT OF CLEAVAGE EVENTS:

1B07_HUMAN
P01889 - HLA class I histocompatibility antigen, B-7 alpha chain

FUNCTION: Involved in the presentation of foreign antigens to the immune system.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
1B07_HUMAN	GYVDDTQFVR	50	FISV | GYVD

PLOT OF CLEAVAGE EVENTS:

NIPA1_HUMAN
Q7RTP0 - Magnesium transporter NIPA1

FUNCTION: Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+) (By similarity). {ECO:0000250}.

SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. Early endosome {ECO:0000250}. Note=Recruited to the cell membrane in response to low extracellular magnesium. {ECO:0000250}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
NIPA1_HUMAN	AAAAAAAAAGEGAR	8	AAAA | AAAA

PLOT OF CLEAVAGE EVENTS:

CNTN1_HUMAN
Q12860 - Contactin-1

FUNCTION: Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth (By similarity). {ECO:0000250}.

SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Lipid-anchor, GPI- anchor; Extracellular side.

SUBCELLULAR LOCATION: Isoform 2: Cell membrane; Lipid-anchor, GPI- anchor; Extracellular side.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
CNTN1_HUMAN	SAQDAPSEAPTEVGVK	803	AVIN | SAQD

PLOT OF CLEAVAGE EVENTS:

PCDH7_HUMAN
O60245 - Protocadherin-7

SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
PCDH7_HUMAN	HIPLTQDIAGDPSYEISK	858	ARSL | HIPL
PCDH7_HUMAN	AVDGGDPPR	714	FRVK | AVDG

PLOT OF CLEAVAGE EVENTS:

PCYOX_HUMAN
Q9UHG3 - Prenylcysteine oxidase 1

FUNCTION: Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine.

SUBCELLULAR LOCATION: Lysosome.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
PCYOX_HUMAN	GCPEGAELR	23	LCSC | GCPE

PLOT OF CLEAVAGE EVENTS:

APOE_HUMAN
P02649 - Apolipoprotein E

FUNCTION: Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. {ECO:0000303|PubMed:3283935}.

SUBCELLULAR LOCATION: Secreted {ECO:0000303|PubMed:3283935}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
APOE_HUMAN	TVGSLAGQPLQER	212	VRAA | TVGS

PLOT OF CLEAVAGE EVENTS:

AMRP_HUMAN
P30533 - Alpha-2-macroglobulin receptor-associated protein

FUNCTION: Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway. {ECO:0000269|PubMed:7774585}.

SUBCELLULAR LOCATION: Rough endoplasmic reticulum lumen {ECO:0000269|PubMed:7774585}. Endoplasmic reticulum-Golgi intermediate compartment lumen {ECO:0000269|PubMed:7774585}. Golgi apparatus, cis-Golgi network {ECO:0000269|PubMed:7774585}. Golgi apparatus lumen {ECO:0000269|PubMed:7774585}. Endosome lumen {ECO:0000269|PubMed:7774585}. Cell surface {ECO:0000269|PubMed:11384978}. Note=May be associated with receptors at the cell surface. {ECO:0000269|PubMed:11384978}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
AMRP_HUMAN	SHQGYSTEAEFEEPR	242	LRRV | SHQG

PLOT OF CLEAVAGE EVENTS:

PCDGK_HUMAN
Q9UN70 - Protocadherin gamma-C3

FUNCTION: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.

SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
PCDGK_HUMAN	STVIHYEIPEER	30	LNKA | STVI

PLOT OF CLEAVAGE EVENTS:

LMAN2_HUMAN
Q12907 - Vesicular integral-membrane protein VIP36

FUNCTION: Plays a role as an intracellular lectin in the early secretory pathway. Interacts with N-acetyl-D-galactosamine and high-mannose type glycans and may also bind to O-linked glycans. Involved in the transport and sorting of glycoproteins carrying high mannose-type glycans (By similarity). {ECO:0000250}.

SUBCELLULAR LOCATION: Endoplasmic reticulum-Golgi intermediate compartment membrane {ECO:0000269|PubMed:10444376}; Single-pass type I membrane protein {ECO:0000269|PubMed:10444376}. Golgi apparatus membrane {ECO:0000269|PubMed:10444376}; Single-pass membrane protein {ECO:0000269|PubMed:10444376}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:10444376}; Single-pass type I membrane protein {ECO:0000269|PubMed:10444376}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
LMAN2_HUMAN	DITDGNSEHLK	45	SVTA | DITD

PLOT OF CLEAVAGE EVENTS:

KDEL2_HUMAN
Q7Z4H8 - KDEL motif-containing protein 2

SUBCELLULAR LOCATION: Endoplasmic reticulum lumen {ECO:0000255|PROSITE-ProRule:PRU10138}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
KDEL2_HUMAN	GAPEVLVSAPR	21	LVAA | GAPE

PLOT OF CLEAVAGE EVENTS:

GPC6_HUMAN
Q9Y625 - Glypican-6

FUNCTION: Cell surface proteoglycan that bears heparan sulfate. Putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases (By similarity). Enhances migration and invasion of cancer cells through WNT5A signaling. {ECO:0000250, ECO:0000269|PubMed:21871017}.

SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Lipid-anchor, GPI-anchor {ECO:0000250}; Extracellular side {ECO:0000250}.

SUBCELLULAR LOCATION: Secreted glypican-6: Secreted, extracellular space {ECO:0000250}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
GPC6_HUMAN	SAPENFNTR	355	RSAR | SAPE

PLOT OF CLEAVAGE EVENTS:

TECT3_HUMAN
Q6NUS6 - Tectonic-3

FUNCTION: Part of the tectonic-like complex which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition (By similarity). May be involved in apoptosis regulation. Necessary for signal transduction through the sonic hedgehog (Shh) signaling pathway. {ECO:0000250, ECO:0000269|PubMed:17464193, ECO:0000269|PubMed:22883145}.

SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
TECT3_HUMAN	STAASLTSPR	370	AFQQ | STAA

PLOT OF CLEAVAGE EVENTS:

TMX3_HUMAN
Q96JJ7 - Protein disulfide-isomerase TMX3

FUNCTION: Probable disulfide isomerase, which participates in the folding of proteins containing disulfide bonds. May act as a dithiol oxidase. {ECO:0000269|PubMed:15623505}.

SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:15623505}; Single-pass membrane protein {ECO:0000269|PubMed:15623505}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
TMX3_HUMAN	SSIASEFGVR	88	ATSY | SSIA

PLOT OF CLEAVAGE EVENTS:

COX5A_HUMAN
P20674 - Cytochrome c oxidase subunit 5A, mitochondrial

FUNCTION: This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

SUBCELLULAR LOCATION: Mitochondrion inner membrane.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
COX5A_HUMAN	SHGSQETDEEFDAR	42	VRCY | SHGS

PLOT OF CLEAVAGE EVENTS:

RTN4R_HUMAN
Q9BZR6 - Reticulon-4 receptor

FUNCTION: Receptor for RTN4, OMG and MAG (PubMed:12037567, PubMed:12068310, PubMed:12426574, PubMed:12089450, PubMed:16712417, PubMed:18411262, PubMed:12839991). Functions as receptor for the sialylated gangliosides GT1b and GM1 (PubMed:18411262). Besides, functions as receptor for chondroitin sulfate proteoglycans (By similarity). Can also bind heparin (By similarity). Intracellular signaling cascades are triggered via the coreceptor NGFR (PubMed:12426574). Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton (PubMed:16712417, PubMed:22325200). Mediates axonal growth inhibition (PubMed:12839991). Plays a role in regulating axon regeneration and neuronal plasticity in the adult central nervous system. Plays a role in postnatal brain development. Required for normal axon migration across the brain midline and normal formation of the corpus callosum. Protects motoneurons against apoptosis; protection against apoptosis is probably mediated via interaction with MAG. Acts in conjunction with RTN4 and LINGO1 in regulating neuronal precursor cell motility during cortical development. Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development (PubMed:22325200). {ECO:0000250|UniProtKB:Q99PI8, ECO:0000269|PubMed:12037567, ECO:0000269|PubMed:12426574, ECO:0000269|PubMed:12839991, ECO:0000269|PubMed:14966521, ECO:0000269|PubMed:16712417, ECO:0000269|PubMed:18411262}.

SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12426574, ECO:0000269|PubMed:12694398, ECO:0000269|PubMed:12839991, ECO:0000269|PubMed:16712417, ECO:0000269|PubMed:18411262}; Lipid- anchor, GPI-anchor {ECO:0000269|PubMed:12694398}. Membrane raft {ECO:0000269|PubMed:12694398}. Cell projection, dendrite {ECO:0000250|UniProtKB:Q99PI8}. Cell projection, axon {ECO:0000250|UniProtKB:Q99PI8}. Perikaryon {ECO:0000250|UniProtKB:Q99M75}. Note=Detected along dendrites and axons, close to synapses, but clearly excluded from synapses. {ECO:0000250|UniProtKB:Q99PI8}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
RTN4R_HUMAN	SVLEPGRPASAGNALK	345	ADKA | SVLE

PLOT OF CLEAVAGE EVENTS:

S39A6_HUMAN
Q13433 - Zinc transporter ZIP6

FUNCTION: May act as a zinc-influx transporter. {ECO:0000269|PubMed:12839489}.

SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12839489}; Multi-pass membrane protein {ECO:0000269|PubMed:12839489}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
S39A6_HUMAN	AAFPQTTEK	27	ELKA | AAFP
S39A6_HUMAN	AFPQTTEK	28	LKAA | AFPQ

PLOT OF CLEAVAGE EVENTS:

NCAM1_HUMAN
P13591 - Neural cell adhesion molecule 1

FUNCTION: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

FUNCTION: (Microbial infection) Acts as a receptor for rabies virus. {ECO:0000269|PubMed:9696812}.

SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein.

SUBCELLULAR LOCATION: Isoform 2: Cell membrane; Single-pass type I membrane protein.

SUBCELLULAR LOCATION: Isoform 3: Cell membrane; Lipid-anchor, GPI- anchor.

SUBCELLULAR LOCATION: Isoform 4: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-anchor {ECO:0000305}.

SUBCELLULAR LOCATION: Isoform 5: Secreted.

SUBCELLULAR LOCATION: Isoform 6: Secreted {ECO:0000305}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
NCAM1_HUMAN	NAPTPQEFR	122	LMFK | NAPT

PLOT OF CLEAVAGE EVENTS:

CNTP2_HUMAN
Q9UHC6 - Contactin-associated protein-like 2

FUNCTION: Required, with CNTNAP1, for radial and longitudinal organization of myelinated axons. Plays a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Demarcates the juxtaparanodal region of the axo-glial junction. {ECO:0000250|UniProtKB:Q9CPW0}.

SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q9CPW0}; Single-pass type I membrane protein {ECO:0000305}. Cell projection, axon {ECO:0000250|UniProtKB:Q9CPW0}. Cell junction, paranodal septate junction {ECO:0000250|UniProtKB:Q9CPW0}. Note=Expressed in the juxtaparadonal region. {ECO:0000250|UniProtKB:Q9CPW0}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
CNTP2_HUMAN	SADFPYNPGQGQAIR	1240	LDSA | SADF

PLOT OF CLEAVAGE EVENTS:

SDF2_HUMAN
Q99470 - Stromal cell-derived factor 2

SUBCELLULAR LOCATION: Secreted {ECO:0000305}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
SDF2_HUMAN	SSLGVVTCGSVVK	19	AVGA | SSLG

PLOT OF CLEAVAGE EVENTS:

P4HA1_HUMAN
P13674 - Prolyl 4-hydroxylase subunit alpha-1

FUNCTION: Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

SUBCELLULAR LOCATION: Endoplasmic reticulum lumen.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
P4HA1_HUMAN	GVAVDYLPER	277	PKKK | GVAV

PLOT OF CLEAVAGE EVENTS:

S38AA_HUMAN
Q9HBR0 - Putative sodium-coupled neutral amino acid transporter 10

FUNCTION: Putative sodium-dependent amino acid/proton antiporter. {ECO:0000250}.

SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
S38AA_HUMAN	SAQDPVVAVAEDGR	429	AARL | SAQD

PLOT OF CLEAVAGE EVENTS:

GPC4_HUMAN
O75487 - Glypican-4

FUNCTION: Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system (By similarity). {ECO:0000250}.

SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Lipid-anchor, GPI-anchor {ECO:0000250}; Extracellular side {ECO:0000250}.

SUBCELLULAR LOCATION: Secreted glypican-4: Secreted, extracellular space {ECO:0000250}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
GPC4_HUMAN	SISESAFSAR	355	RISR | SISE

PLOT OF CLEAVAGE EVENTS:

LAMC1_HUMAN
P11047 - Laminin subunit gamma-1

FUNCTION: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
LAMC1_HUMAN	SQAAQEAEINAR	1493	AGMA | SQAA

PLOT OF CLEAVAGE EVENTS:

ATRN_HUMAN
O75882 - Attractin

FUNCTION: Involved in the initial immune cell clustering during inflammatory response and may regulate chemotactic activity of chemokines. May play a role in melanocortin signaling pathways that regulate energy homeostasis and hair color. Low-affinity receptor for agouti (By similarity). Has a critical role in normal myelination in the central nervous system (By similarity). {ECO:0000250, ECO:0000269|PubMed:9736737}.

SUBCELLULAR LOCATION: Isoform 1: Cell membrane {ECO:0000269|PubMed:7539799}; Single-pass type I membrane protein {ECO:0000269|PubMed:7539799}.

SUBCELLULAR LOCATION: Isoform 2: Secreted {ECO:0000269|PubMed:7539799, ECO:0000269|PubMed:9736737}.

SUBCELLULAR LOCATION: Isoform 3: Secreted {ECO:0000269|PubMed:7539799}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
ATRN_HUMAN	AVSGSAAAEAK	89	AAAA | AVSG

PLOT OF CLEAVAGE EVENTS:

PDIA1_HUMAN
P07237 - Protein disulfide-isomerase

FUNCTION: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307). {ECO:0000269|PubMed:10636893, ECO:0000269|PubMed:12485997, ECO:0000269|PubMed:21670307}.

SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000269|PubMed:23475612}. Endoplasmic reticulum lumen {ECO:0000269|PubMed:10636893, ECO:0000269|PubMed:23475612}. Melanosome {ECO:0000269|PubMed:12643545, ECO:0000269|PubMed:17081065}. Cell membrane {ECO:0000269|PubMed:21670307}; Peripheral membrane protein {ECO:0000305}. Note=Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces (PubMed:11181151). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:10636893). Colocalizes with MTTP in the endoplasmic reticulum (PubMed:23475612). {ECO:0000269|PubMed:10636893, ECO:0000269|PubMed:11181151, ECO:0000269|PubMed:23475612, ECO:0000305}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
PDIA1_HUMAN	NFEDVAFDEK	376	LVGK | NFED

PLOT OF CLEAVAGE EVENTS:

BCLF1_HUMAN
Q9NYF8 - Bcl-2-associated transcription factor 1

FUNCTION: Death-promoting transcriptional repressor. May be involved in cyclin-D1/ND1 mRNA stability through the SNARP complex which associates with both the 3'end of the ND1 gene and its mRNA. {ECO:0000269|PubMed:18794151}.

SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Nucleus speckle {ECO:0000269|PubMed:24100041}. Nucleus, nucleoplasm {ECO:0000269|PubMed:24100041}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
BCLF1_HUMAN	SAAMTLNER	623	QYFK | SAAM

PLOT OF CLEAVAGE EVENTS:

LYOX_HUMAN
P28300 - Protein-lysine 6-oxidase

FUNCTION: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin (PubMed:26838787). Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture (By similarity). {ECO:0000250|UniProtKB:P28301, ECO:0000269|PubMed:26838787}.

SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P28301}. Secreted, extracellular space.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
LYOX_HUMAN	SLGSQYQPQR	59	FSLL | SLGS

PLOT OF CLEAVAGE EVENTS:

CO2A1_HUMAN
P02458 - Collagen alpha-1(II) chain {ECO:0000305}

FUNCTION: Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix {ECO:0000255|PROSITE-ProRule:PRU00793}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
CO2A1_HUMAN	SLNNQIESIR	1261	ATLK | SLNN

PLOT OF CLEAVAGE EVENTS:

SYVM_HUMAN
Q5ST30 - Valine--tRNA ligase, mitochondrial

SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
SYVM_HUMAN	SVSTQSEPHGSPISR	27	PRFH | SVST

PLOT OF CLEAVAGE EVENTS:

AMD_HUMAN
P19021 - Peptidyl-glycine alpha-amidating monooxygenase

FUNCTION: Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.

SUBCELLULAR LOCATION: Isoform 1: Membrane; Single-pass type I membrane protein.

SUBCELLULAR LOCATION: Isoform 2: Membrane; Single-pass type I membrane protein.

SUBCELLULAR LOCATION: Isoform 3: Secreted. Note=Secreted from secretory granules.

SUBCELLULAR LOCATION: Isoform 4: Secreted. Note=Secreted from secretory granules.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
AMD_HUMAN	GIEVQEIK	821	VKKA | GIEV

PLOT OF CLEAVAGE EVENTS:

MARC1_HUMAN
Q5VT66 - Mitochondrial amidoxime-reducing component 1

FUNCTION: As a component of an N-hydroxylated prodrug-converting complex required to reduce N-hydroxylated prodrugs, such as benzamidoxime. Also able to reduce N(omega)-hydroxy-L-arginine (NOHA) and N(omega)-hydroxy-N(delta)-methyl-L-arginine (NHAM) into L-arginine and N(delta)-methyl-L-arginine, respectively. {ECO:0000269|PubMed:19053771}.

SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000269|PubMed:23086957}; Single-pass type II membrane protein {ECO:0000269|PubMed:23086957}. Membrane {ECO:0000305}; Lipid-anchor {ECO:0000305}. Note=Mitochondrial import is mediated by AA 1-40 and requires ATP.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
MARC1_HUMAN	GAAGSSALAR	2	#NAME? | GAAG

PLOT OF CLEAVAGE EVENTS:

PTPRS_HUMAN
Q13332 - Receptor-type tyrosine-protein phosphatase S

FUNCTION: Cell surface receptor that binds to glycosaminoglycans, including chondroitin sulfate proteoglycans and heparan sulfate proteoglycan (PubMed:21454754). Binding to chondroitin sulfate and heparan sulfate proteoglycans has opposite effects on PTPRS oligomerization and regulation of neurite outgrowth. Contributes to the inhibition of neurite and axonal outgrowth by chondroitin sulfate proteoglycans, also after nerve transection. Plays a role in stimulating neurite outgrowth in response to the heparan sulfate proteoglycan GPC2. Required for normal brain development, especially for normal development of the pituitary gland and the olfactory bulb. Functions as tyrosine phosphatase (PubMed:8524829). Mediates dephosphorylation of NTRK1, NTRK2 and NTRK3 (By similarity). Plays a role in down-regulation of signaling cascades that lead to the activation of Akt and MAP kinases (By similarity). Down-regulates TLR9-mediated activation of NF-kappa-B, as well as production of TNF, interferon alpha and interferon beta (PubMed:26231120). {ECO:0000250|UniProtKB:B0V2N1, ECO:0000250|UniProtKB:F1NWE3, ECO:0000269|PubMed:21454754, ECO:0000269|PubMed:26231120, ECO:0000269|PubMed:8524829}.

SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:26231120}; Single-pass type I membrane protein {ECO:0000305}. Cell projection, axon {ECO:0000250|UniProtKB:B0V2N1}. Perikaryon {ECO:0000250|UniProtKB:B0V2N1}. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250|UniProtKB:Q64605}. Cell junction, synapse, synaptosome {ECO:0000250|UniProtKB:Q64605}. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250|UniProtKB:Q64605}. Note=Is rapidly internalized when dendritic cells are stimulated with the TLR9 ligand cytidine- phosphate-guanosine (CpG) (PubMed:26231120). Detected in a punctate pattern along neurites and axon growth cones (By similarity). {ECO:0000250|UniProtKB:B0V2N1, ECO:0000269|PubMed:26231120}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
PTPRS_HUMAN	SIPEDTPR	922	AEVL | SIPE

PLOT OF CLEAVAGE EVENTS:

PDPN_HUMAN
Q86YL7 - Podoplanin

FUNCTION: May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Ligand for CLEC1B, a platelet receptor (PubMed:18215137). Induces platelet aggregation (PubMed:14522983, PubMed:15231832). Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. {ECO:0000250|UniProtKB:Q62011, ECO:0000269|PubMed:14522983, ECO:0000269|PubMed:15231832, ECO:0000269|PubMed:15515019, ECO:0000269|PubMed:18215137, ECO:0000269|PubMed:9651190}.

SUBCELLULAR LOCATION: Membrane {ECO:0000305|PubMed:18215137}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q62011}. Cell projection, lamellipodium membrane {ECO:0000250|UniProtKB:Q62011}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q62011}. Cell projection, filopodium membrane {ECO:0000250|UniProtKB:Q62011}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q62011}. Cell projection, microvillus membrane {ECO:0000250|UniProtKB:Q62011}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q62011}. Cell projection, ruffle membrane {ECO:0000250|UniProtKB:Q62011}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q62011}. Note=Localized to actin- rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles. {ECO:0000250|UniProtKB:Q62011}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
PDPN_HUMAN	SVNSVTGIR	71	LVAT | SVNS

PLOT OF CLEAVAGE EVENTS:

HACD2_HUMAN
Q6Y1H2 - Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 {ECO:0000305}

FUNCTION: Catalyzes the third of the four reactions of the long- chain fatty acids elongation cycle. This endoplasmic reticulum- bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. {ECO:0000269|PubMed:18554506}.

SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:15024066, ECO:0000269|PubMed:18554506}; Multi- pass membrane protein {ECO:0000269|PubMed:15024066, ECO:0000269|PubMed:18554506}.

CLEAVAGE EVENTS OBSERVED:

Protein	Peptide	Cleavage Position (P1')	Cleavage Site P4-P1 | P1'-P4'
HACD2_HUMAN	AAVAATAAAK	2	#NAME? | AAVA

PLOT OF CLEAVAGE EVENTS: